Any researcher has this antibody for Immunohistochemistry TRPC5?

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How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

Hello all Can we use different concentrations of lemon or orange extract to grow flowers in ornamental trees and shrubs?

I need more pdf on this topic

02 July 2024 6,988 1 View

Is there a close relationship between the glassbrush bush and its insect pollinator, the honey bee?

I need more information about this topic

27 June 2024 4,057 3 View

How do bees work on glassbrush flowers?

I need pdf information to increase my knowledge of this pollinator, which is honeybees

27 June 2024 3,815 1 View

Welcome What is the scientific name of this climbing plant?

I was confused about the scientific name as well as the local name because of the great similarity between this plant and another climbing plant, especially the morphological shape, which is very...

22 June 2024 9,570 2 View

Hello all Do peanut shell residues preserve soil moisture after planting?

I want to confirm the information so I can add it to my simple information

20 June 2024 4,160 0 View

Welcome Find these basic axes in p df form:1.Introduction to Botany:2.Plant Structure and Function?

conservation Conclusion: In summary, "Exploring Botany: An Inclusive Guide to Plant Biology" aims to be an invaluable resource for students, educators, and enthusiasts interested in delving into...

13 June 2024 5,807 1 View

Hello all I am currently working in extracting plant parts using ultrasound. Can I use the GC-MS technology or chromatographic analysis?

A number of active compounds and their concentrations found in flower and leaf extracts were obtained using this technique

09 June 2024 1,872 4 View

Why does the aloe vera gel change color from white to pink when placed in the refrigerator?

While when placed in the freezer, it maintains the same original white color?

05 June 2024 5,231 5 View

Welcome Do I need a free Scopus indexed magazine?

I have an English research paper that I need to publish

05 June 2024 6,772 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Saroj Kumar Khan

Abc kit applied for primary antibody,fluorescence antibody colourometric antibody and conjugated.

Epitope is prima facie.

Tumor necrosis factor TNF alpha1

Etearnarcept Efor hmn 1 and 2 TNF -TNFR1.

LT2-TNFR2 cross also TNF TNFR2 and And LT2- TNFR1

Cypzox is best medicine 💊 for these arthritis various.