21 June 2023 2 8K Report

For mouse

More Fatma Fathi's questions See All
Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Bubble column design?

A bubble column of 10 m height and 1m diameter (implying high hydrostatic pressure difference) is being fed leisurely with a flow rate of 10 L/min. What is the resulting dissolved oxygen profile...

31 December 2020 524 1 View

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can anyone know the range of the osteoclast activity range (resorption) in bone remodeling cycle in mice compared to humans. or any paper talking about this information. I need this specific...

14 September 2020 1,218 2 View

What is the most reliable method for performing ELISA test on mouse brain?

Dear fellow researchers, may I ask you for your advice regarding performing ELISA test on mouse brain? which method gives a better yield snap freezing the tissue in liquid nitrogen, storing it...

26 July 2020 1,376 3 View

Help for interpretation ?

I did a survey and i found that 21 of people are unemployed , 14 are retired, when 24 are workers , what that means in terms of sustainability ? , the number of people that replied to my...

08 June 2020 7,785 2 View

What is meant by awaiting reviewer selection after being under review in scholar one online submission system?

I have a submitted a manuscript The status was for two month under review then turned awaiting reviewer selection

31 May 2020 688 3 View

Similar questions and discussions
How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

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