I am investigating the presence of metal ions (Cr, Ni, Cu, Fe, and Mo) in an alkaline electrolyte (pH>11)and would like to use UV-Vis spectrophotometry for their detection and quantification.
Could anyone recommend:
- Suitable complexing agents or chromogenic reagents for metal( Cr, Ni, Cu, Fe, and Mo) that work well in alkaline pH?
- Wavelengths or absorption maxima for these metal complexes?
- Any sample preparation or pH adjustment steps needed to improve sensitivity or selectivity?
- Known interferences or matrix effects in alkaline solutions?
If you have experience with single or multi-element detection using UV-Vis in similar systems, I’d greatly appreciate your insights or references to relevant protocols or literature.
Stefhanya Ormeño
Yes, several UV-Vis spectrophotometric methods are available for metal ion detection in alkaline media, though most require complexation with specific chromogenic reagents. For example:
- Cr(VI): Use of 1,5-diphenylcarbazide in alkaline or slightly acidic media (λ ~540 nm).
- Ni(II): Complexation with dimethylglyoxime under alkaline conditions (λ ~445 nm).
- Cu(II): Bicinchoninic acid (BCA) or neocuproine in alkaline pH (λ ~562 nm or 450 nm).
- Fe(III): Thiocyanate complexation (λ ~480 nm), though more stable under acidic pH, it can be buffered for alkaline systems.
- Mo(VI): Complex with thiocyanate or 4-(2-pyridylazo)resorcinol (PAR), stable at slightly basic pH.
Always ensure pH adjustment and masking of interfering ions when analyzing mixtures.
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