Fungal testing typically includes a microscopic examination of the sample on a slide, sometimes using a preparation or stain to aid in detection of fungal elements. Blood tests are often used to diagnose more serious fungal infections.

Dear Madhukar.

I suggest you review the book entitled (Diagnosis and idetification of plant pathogens edited by H.-W. Dehne etal in which you will find several chapters dealing with this subject.

Best Regard

Hi, Poonam Gusain ,

These days I am working on isolation and identification of fungi. I agree with the reply of Dr Madhukar Baburao Deshmukh here because what we usually do for identification has two main steps. First one is to observe the fungus under the microscope to observe specific structure like conidia, conidiophore or fruiting bodies, sexual or asexual structures. Then we record these specific features and go through fungal identification keys which are already published for fungal morphological identification. The second step is to do molecular biological identification using a proper primer. Other than that we do antimicrobial assays(spot testing, well diffusion assays) for fungi as well. Moreover, there are some biochemical activity tests I have done for fungi on culture plates to characterize their enzyme production (e.g. amylase production, cellulase production, gelatinase). Therefore, I think you can decide what tests you should do for your fungi according to your requirement. I also think the best way to identify a novel fungi is to do some molecular studies along with basic morphological identification before that

I know these two traits are very common for identifying fungi, for knowing metabolic features of novel fungi, can we have some biochemical screening methods: I hope you will be getting my point now. Thank you Sandhya Jayasekara for your answer.