maybe by checking pH constantly.

You can find what you need in the FDA draft guidance for Doxorubicin HCl:

Doxorubicin hydrochloride (fda.gov)

https://www.accessdata.fda.gov/drugsatfda_docs/psg/Doxorubicin%20Hydrochloride_draft_Injection%20injec%20lipo_RLD%2050718_RC09-18.pdf

It depends on your end goal.

If you want to break the particles rapidly, you can try sonication.

If you want to evaluate slow drug release, you can try dialysis.

Dialysis is commonly used to evaluate the drug release profile of liposomal dox. You can also try chromatographic techniques.

Thank you all for your reply.

Muhammad Saad Khan thank you.

May I know which buffer to be used for sonication?