Have you ever seen phycocyanin content higher than 20% (w/w) and what about the C/N ratio to improve its synthesis ?
Thanks in advance for yours answers
Dear Nicolas, the content of phycocyanins in Spirulina usually exceeds 10% (w/w). I think that your result may depend on the purification method of phycocyanins that you adapted for your protocol. Please see the attached paper. I believe that it may help you to clarify any doubts. Also, I am sending you papers that describe the average composition of lyophilised Spirulina biomass.
Dear Nicolas, Please see another paper which describes phycocyanin synthesis in two different growth media by various Spirulina strains.
Dear Lukeasz,
Many thanks for your answer and attached files.
A couple of additional questions : do you think that Phycocynain degradation peptides may be responsibale of higher contents (> 15%, based on Patel et al. 2005 formulae) ? Have you ever measured phycocyanin degradation by SDS PAGE densitomtry ? Any correlation with Abs measurment at 620 and 650 nm ?
Thanks in advance.
Bonjour Nicolas,
ces rendements sont facilement atteints sur des biomasse non déshydratées. Pour des extractions sur poudre sèche, la valeur est plutôt deux fois moindre (~10%).
La teneur en phycocyanine et la composition des antennes PC/APC/PE ne dépend pas uniquement du C/N. C'est aussi, pour une variété donnée, une question de quantité et de qualité de la lumière.
Au plaisir
I agree with Jean-Francois Sassi, that the contents of Phycocynain not depend on C/N.
Dear Nicolas, Sorry for my late response. I am going to carry out some tests on isolation of phycobilibroteins in the next few weeks and I will update you how it goes. Personally, I dont' think that SDS PAGE is necessary - it was only used to verify whether purification method offered by authors was effective. You need to follow all other purification steps though, including DEAE-Sepharose chromatography, and carry out measurements using spectrophotometer.
To all, thanks for yours answers
To Lukasz,
Yes, please let me know your ongoing experiments.
What is the best phycocyaniin content you´ve ever obtained in the crude extract ? Do you think that Patel et al. method is enough to quantify phycocyanin in the crude extract ? And will you run Total protein assay during your purification ?
Many thanks in advances for your fedback
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