No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

How to develop a framework/model and present it in an analytical form?

Dear All, I am conducting a study on DBD actuators and I'm trying to develop a framework for actuators using plasma-based metamaterials. In order to reach this objective, I need to learn how to...

01 June 2020 3,864 2 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Solution not converged at time ........... in ansys?

I am doing four point flexural text on simply supported beam using ANSYS APDL first with steel plate for loading and supporting and then without it. In both cases there is a problem of non...

19 March 2020 4,775 6 View

Can we find the Link between Teaching Methods and Achievement in Math in Computer-Assisted Elementary Schools?

I published a paper in: Creative Education, Published Online http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47205 The Link between Teaching Methods and Achievement in Math in...

14 February 2020 1,408 4 View

How to detrend a series in Stata when it has a stochastic trend?

08 February 2020 1,803 3 View

What is the editorial office email of this journal?

I submitted an article and received an email stating that I need to fix the word count to 5000 or less., though the word count in my manuscript is 4330. I sent back an email in this regard and...

06 October 2019 9,154 3 View

Is an encrypted dataset with Microsoft SEAL compatible with Cloudsim simulator??

Hello, I would like to do homomorphic encryption using Microsoft SEAL on my dataset and then use it as an input to the Cloudsim simulator to do clustering on. Is it possible and compatible with...

16 February 2021 3,915 1 View

How can analyze the results in the cloudsim simulator?

I am looking for a way to analyze the results in the cloudsim simulator.

02 January 2021 1,192 2 View

How do I turn off the wall function for k-w SST simulation in Ansys Fluent?

I am using a very fine mesh (D/1000). Since I cannot find an option to turn off the wall function in Fluent GUI, could you please let me know how I can turn it off? From some forum I got to know...

21 December 2020 9,186 15 View

Fluent simulation gets diverged in Batch Mode but converged in GUI mode?

Hi, I did a combustion simulation using the species transport model in my PC where Ansys 2020R2 is installed. I used a Chemkin mechanism and Chemkin-CFD solver for the chemistry part. The...

20 December 2020 168 2 View

How does cloudsim clock operate?

I read a lot of papers on cloudsim but I don't get how cloudsim's clock actually works.at the end of the simulations it's always showing 10000.0 and it's not clear whether it operates in...

19 November 2020 7,371 3 View

What do printed execution times and application loop delays represent in ifogsim?

hi I'm working on ifogsim and I've run some of the examples provided (DCNSFog.java) but I don't get what these printed time results mean. to be clear in the below image execution time is 3007 ms....

05 November 2020 2,417 3 View

Is there a way to make Facio (GUI for FMO) work on Mac OS Catalina?

I am trying to use the FMO (Fragment Molecular Orbital) method which works to make large (thousands of atoms) molecules more palatable for ab initio computational quantum-chemical calculations on...

29 September 2020 4,896 2 View

Is there a way to calculate energies for all residues from a pdb?

CHARMM GUI online does a protein-protein interaction where you can measure the interaction energy of one chain with all the others. Is there a way to do this for each residue? Basically I want to...

29 July 2020 8,851 3 View

Best package for statistical power analysis in R?

Hi, I have used the package pwr in R, but it falls short when compared with the GUI program G*Power (https://gpower.hhu.de/). G*Power has a wealth of options and analyses but pwr is more...

22 July 2020 3,753 3 View

Problem downloading Sentinel-2 Offline data ?

I am trying to download Sentinel-2 data of year 2018 over Hong Kong and the Pearl River Estuary. But as 2018 data is offline I am neither able to download it using python batch processing script...

05 April 2020 7,852 6 View