I have a blood-isolate, from a pneumonia patient, which is like that, identification confirmed with conventional as well as bacterial antigen, API and PCR testing methods.
Its can grow without co2 (need 3 day for give good isolate )...... But if you use co2 5% by jar it will grow more speed ( need 1 day nearly )
http://www.google.iq/url?sa=t&rct=j&q=Streptococcus+pneumoniae+growing+anaerobicallyarbon+dioxide&source=web&cd=5&ved=0CGgQFjAE&url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC475944%2F&ei=6K0bULm7EaSV0QW47oHoCw&usg=AFQjCNH2MgaEFx6rXm1dS0bbxjia4A6oTw
it is a fastidious bacteria and takes 2-3 days to grow but its growth is more rapid if u provide CO2. but it is not strictly anaerobic
It was incubated for 5 days in CO2, but no growth on the plates, while it grew after 48 hours on the anaerobically incubated plates.
This strain of S.pneumoniae which is behaving as a strict anaerobe, may grow in CO2 if you try repeated subcultures in CO2. Initially the colonies may be very tiny. On further subculturing it may adapt to CO2 and later under O2 condition. It is worth trying.
Thanks Dr. Molly. We had incubated the plates in CO2 for 5 days, but absolutely no growth at all!
Maybe your bacteria is very sensitive so try keep ur bacteria incubate with CO2 only max 3 days or twice subculture in a week. If you want to keep longer i suggest you used microbead or put in Bactec Blood Culture (BD Company) aerobic and anaerobic. See what happen?
This is my practices in culturing bacteria in USM.
Thanks Anizan. It had originally grown in the Bactec bottle (anaerobic) only, problem was in growing it in subcultures for identification and susceptibility.
Strictly anaerobic we haven't seen them, though capnophilic they certainly can be.
picking it up by antigen, API and PCR method is still possible with homologous species. What PCR did you use? if it is LytA is becomes more plausible it is S. pneumonia indeed. If it is pneumolysin or capsule it might be different. If LytA positive I would try a more rich or different broth(s) to culture in or double blood plates (like 10% sheep blood) which seems to help sometimes sinificantly.
Thanks a lot for the insight Debby. I'll find out what PCR they have used, as its done in our molecular reference lab. I'll try using 10% sheep/horse blood with the stocked strain. Thanks once again.
I have seen several isolates of Strep pneumo that only grow anaerobically. I have also seen them "auto lyse " if not sub cultured from positive blood culture agar in a timely manner. many times I have seen picture perfect diagnostic strep pneumo in sputum gram stains along with many white cells that we were unable to grow. So I know some stains will only grow anaerobically
which medium you are using? Try using Tood Hewitt broth supplement with sheep blood and agar as medium for culturing.
Using the normal 5% sheep blood agar, and trypticase soy broth as the liquid medium. I'll try as you have suggested.
We use oxyrase with BHI or THY for anaerobic cultivation of pneumococci. Very easy and quick method. https://www.oxyrase.com/.
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