i'm getting 190bp PCR-RFLP band, can you help which I couldn't find in any other research paper related to the subject and also I'm getting another 200bp band which is also new for me.
Further question, is the C allele has a 212bp band, because I'm still confirming?
Kind Regards
Dear student,
to answer your question I should have more information: which gene and which region of DNA are you looking at? I suppose CSN1S1. If so, the C allele CSN1S1 should be characterized by a band of 223 bp.
Kind Regards
RAMUNNO L., COSENZA G., RANDO A, PAUCIULLO A, ILLARIO R., GALLO D, DI BERARDINO D., MASINA P. (2005). Comparative analysis of gene sequence of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland. Gene, 345 (2), 289-299. ISSN: 0378-1119, doi: 10.1016/j.gene.2004.12.003; ISI: 000227363200015; SCOPUS: 2-s2.0-13644266892
RAMUNNO L., COSENZA G., PAPPALARDO M., PASTORE N., GALLO D., DI GREGORIO P., MASINA P. (2000). Identification of the goat CSN1S1F allele by means of PCR-RFLP method. Animal Genetics, 31 (5), 342-3.ISSN: 1365-2052, doi: 10.1046/j.1365-2052.2000.00663.x; ISI: 000165797800018; SCOPUS: 2-s2.0-0033625991
Erratum. Animal Genetics 32 (1), 54-54. ISI: 000169325000020; SCOPUS: 2-s2.0-0034965790
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