which material is used as base for thin film sample preparation in XRD analysis?

kindly guide me to prepare a Thin-film sample of nanoparticles for XRD analysis.

10 January 2021 3,744 4 View

Can the cell line NG108-15 (neuroblastoma x glioma hybrid) form synapses and show network connectivity?

Does it form networks in 3D cell culture (e.g. when grown in collagen matrix)? What other options for neural cell lines (PNS related) exist that show network connectivity? Can the network...

17 September 2020 4,923 2 View

I need help to interpret my cox proportional model?

Hi There I was performing a cox proportional model for a cancer study in SPSS and I had this output where the Exp(B) was 0.0000064 and the CI 0 to 3.69E+51 ; p= 1 How would I report this Thank...

13 August 2019 5,054 3 View

QuPath: How to measure intensity of staining?

Hi, I'm currently working on my steroid receptor project. I have different receptors stained in tissue samples and currently using QuPath programme to analyze the scanned images. My question is,...

15 June 2019 6,489 3 View

Hello, How can I use the D-R and Tempkin isotherm models on the Origin Pro software? How write the equation and use?

Mostly I use the Langmuir, Freundlich and Sips model for the describe the isotherm database but I don't know how to use D-R and Tempkin isotherm models

20 March 2019 4,106 15 View

Whether I need to adjust the PH of percoll used for gradient centrifugation?

Hi, I am going to do Percoll gradient centrifugation for subcellular fractionation. Before I start I would like to know whether I need to adjust the PH of percoll to 7.4. I use percoll from...

19 November 2018 2,393 3 View

Can we changed the manuscript title when it comes to us back for revision?

I have received revision for a manuscript i submitted to a journal...I had done a survey among elderly population in randomly selected 4 districts...the sample size was 600 and was selected from...

26 May 2018 4,188 3 View

How to generalize a survey done for a specific sample?

I have done a survey among 600 elderly living in old age homes. The sample size was estimated using prevalence of previous study and the sample was selected using cluster random sampling. 4...

25 May 2018 2,494 4 View

Is immunoflorescence staining or western blot is more sensitive to study the protein expression?

Is immunofluorescence staining or western blot is more sensitive to study the protein expression?

25 March 2018 5,866 8 View

Can anyone help me with implementing fuzzy SVM in Python?

Can anyone help me implementing fuzzy SVM in Python or any other language? I want to see if the fuzzified SVM yeilds better results than naive SVM for my dataset.

06 March 2018 5,999 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View