Anusuya - FAQS.TIPS

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What are the research scope of cloud forensics. please mention the organizations who are doing active research in cloud forensics ?

cloud forensics

23 June 2024 9,811 1 View

What is equivalent energy of biofertilizers? Or how can we calculate equivalent energy of organic manure input?

What is equivalent energy of biofertilizers (rhizobium, PSB, KSB, etc)? Or how can we calculate equivalent energy of organic manure (FYM, neem cake manure, etc) input?

08 September 2023 8,898 1 View

Reason for double RE digestion not working?

I have a insert of 400 bp cloned in vector pbluescript II KS (+) of size 3.0kb at RE sites XbaI and XhoI. But when I try to double digest the plasmid it is not happening. I am sharing picture of...

10 August 2023 3,245 3 View

What is the concept of soil mass depth (Kg/m²)?

I am working on 137Cs-based soil erosion estimation. For this purpose, I need to calculate the soil mass depth (Kg/m²). Suppose I am using a box monolith with dimensions of 0.15 meters in length...

21 July 2023 6,171 1 View

Why GST-tagged recombinant protein containing BL21 cell become viscous after three cycle of freeze thaw at -80 degree Celsius?

i was cloned the lysin gene of bacteriophage in the pGEX 4t1 vector and transformed them in the BL21 Competent cell. competent cell was transformed and upon induction they are showing the...

03 July 2023 6,890 1 View

How to change the elution buffer with tris Cl buffer before cleavage of thioredoxin tag from recombinant protein with enterokinase?

my recombinant vector contain thioredoxin tag and my elution buffer contain imidazole, NaCl and NaH2PO4. so how can i replace my elution buffer with tris-Cl buffer before enteokinase treatment

06 June 2023 4,572 4 View

Why the E.coli cell containing the recombinant cell does not express that particular protein upon induction with iptg?

i am cloning my gene of interest in vector pet32 and pgex4t. upon transformation i got colony on antibiotic containing LB Agar. also colony pcr and pcr with the plasmid extracted from the clone...

22 April 2023 1,167 4 View

Is freeze thaw is sufficient to lyse the e.coli BLA21 Cell containing recombinant protein?

While performing sonication i am facing the issue of frothing which i think results in the aggregation of desired protein and leads to the formation of inclusion bodies

25 February 2023 856 2 View

How do I label the Trichostatin-A with Technetium 99m to study the bio-distribution in mice.?

I attempted to label the Trichostain-A with Technetium 99m in order to study the biodistribution. I ran TLC to analyze the binding but found no labelling. What methods or chemical modification can...

26 December 2022 4,420 0 View

Greetings.I would like to know whether there is any incidence of Citrobacter freundii in spoilage of fish?

Myself Anu Ruby Benny. My research topic is related to identification of specific spoilage bacteria in iced fish.

28 July 2021 1,685 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View