Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?
use antibody modelling suite of schrodinger or moe software. it has own database to suggest you..both softwares are commercial
In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.
06 July 2017 8,853 5 View
I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.
05 June 2017 5,918 4 View
I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...
05 June 2017 373 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷
13 July 2024 3,382 0 View