Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

What is the effect of SDS running buffer concentration on western results?

I am trying to do a thermal shift assay for a recombinantly-produced membrane protein, by western blot band quantification. I use Bio-rad 10X Tris/glycine SDS running buffer for my SDS-PAGE and...

11 January 2021 5,302 2 View

Who are the leading Indigenous academics who have researched and published on STEM teaching and learning?

I'm interested to read what leading Indigenous academics have to say about the intersection between traditional or Indigenous knowledges/epistemologies, values/axiologies and ways of...

14 December 2020 10,026 10 View

Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

No title

I am medical student. I want robbins pathologie fr deutsch pdf . how am I find this book ?

13 December 2020 8,136 3 View

How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

Why does immunofluorescence but not western blot works for fusion protein?

I have a flag-tagged protein and want to be sure it is properly expressing in my cells. When I assess by IF, I see clear nuclear enrichment (expected). But when I do a western, there is only a...

27 November 2020 2,350 3 View

Is there any tool to extract phenotypes from facial photos?

The facial photos corresponds to various rare genetic disorders.

22 November 2020 738 1 View

Could you kindly assist with the journals that are related to my title below?

Effects of covid-19 pandemic on micro companies within the event sector in Western Cape.

08 November 2020 2,138 2 View

Do you know any examples of ethnic stereotypes reflected in literature on wooden architecture in Central and Eastern Europe?

In 19th and 20th centuries wooden architecture became very popular among researchers and enthusiasts of vernacular culture in Central and Eastern Europe. Numerous texsts by professional scholars...

18 October 2020 9,659 2 View