How does SEA software normally number subsystems?

Dear colleagues, What is the common subsystem numbering system in SEA modelling software (i.e. VA One, AutoSEA2)? Cheers, Feng

27 February 2021 7,073 3 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

How to search the specific primer for genus Sphingobium ?

Dear all, I want to find a specific primer for genus Sphingobium, could you give me some idea on it? Thank you so much~

13 December 2020 4,455 4 View

Why case-controlled study is more bias prone than cohort study?

I noticed from several articles saying that case-controlled study is more bias prone than cohort study (in the context of cancer early detection using molecular marker). I spent sometime to try to...

17 November 2020 1,878 4 View

How to get the right result of iwr1443?

05 October 2020 10,015 3 View

Can we compare Bray Curtis dissimilarity indices calculated i) from proportions and ii) raw values ?

Hi everyone! I have a technical question here related to ecological distance indices. I have calculated a Bray-Curtis dissimilarity matrix for several sites using the raw abundance value of...

09 September 2020 2,752 1 View

Why the gold turns from grey to red after sputtering on PTFE?

Hi all, Recently, I sputtered some gold on the surface of PTFE with a thickness of 1 nm and 3 nm (Please see the attached). The color was grey when it was done. However, after about one day, the...

19 August 2020 1,267 3 View

If cell pellet has been stored in -80 for two years. Can RNA be extracted for mi-RNA Seq? Would it be of good quality?

Hello everyone! If cell pellet has been stored in -80 for two years. Can RNA be extracted for mi-RNA Seq? Would it be of good quality? And what's your recommended methods of RNA extraction from...

30 June 2020 3,947 1 View

How to inhibit or supress cracking in Ni-W electraodeposition?

I'm try to synthesize Ni-W alloy using DC electrodeposition. However, plenty of cracks were found in the Ni-W deposits with a relative high W content (approximately 20 wt.%). Is there any...

11 June 2020 442 5 View

I am reading journal paper about mangroves. And I would like to get some recommend journal related to dissolved oxygen influence on mangrove growth?

I am wondering, why my study result show that Kandelia candel and Avicennia alba species are most sensitive towards soil dissolved oxygen (DO) besides R. apiculata among the mangrove species in...

27 May 2020 1,366 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View