I am trying to find an image base tracking program (if possible, open source) that can reliable discern immobility from freezing response in mice while assessing fear conditioning behavioural responses.
Is anything that you can recommend?
Thank you very much!
Hi Marta, open-source software for analyzing many different animal behaviors is often hard to implement for novel behavior paradigms. Based on your criteria of discerning immobility from freezing responses, I recommend you utilize open-source machine learning platforms like DeepLabCut (Mathis Lab) and SIMBA (Simple Behavior Analysis; from Golden's Lab). DeepLabCut is a pose estimation program that can track user-defined parameters (like the mouse's nose, head, thorax, tail, ears, etc.). SIMBA can then take those pose estimates and help you create classifier models for various behaviors you may want (like being able to distinguish freezing behavior from immobility).
Website links are provided below:
SIMBA: https://goldenneurolab.com/simba
DeepLabCut: http://www.mackenziemathislab.org/deeplabcut
I hope this helps.
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