You can try with a Touchdown PCR protocol...

may this will help you in some way

if there is no pcr product: lower the annealing temperature to 2 deg. celcius below the lowest primer annealing temperature. as a rule of thumb AT adds 2 degree and GC 4 deg t o the temp of the sequence. then add 1.5M betaine and 5%betaine and 5% DMSO to the pcr amplification

if no effect is seen increase the betaine concentration to 2.5M add 10%DMSO and double the amount of taq polymerase. the annealing temperature can be further decreased.

if you are seeing many unspecific pcr product: increase the annealing tempand add 1.5M betaine and 5% DMSO. if no effect is seen increase the betaine and DMSO concentration...

also try this

FailSafe™ PCR (http://www.epibio.com/newsletter/f6_2/f6_2failsafe.asp)

Long-Distance PCR (http://nar.oxfordjournals.org/content/24/3/538.full)

@ Nicola @ Yasar... Thx a lot i ll try

deazaGTP