The hydrolysis phase of the biogas production process includes the degradation steps of macromolecules to the individual components and finally to fatty acids, CO 2 and H 2 , but also various intermediates. In these degradation processes, a variety of microorganisms is involved or the degradation takes many steps or reactions.
The products of the hydrolysis phase are a substrate for the methane formation by methanogenic bacteria or archaea. For scientific investigations of a double-stage biogas process, which focused on methanation stage, is it possible to prepare a substrate in the lab instead of operating a hydrolysis stage?
Yes, substrates can be prepared from different sources instead of running hydrolysis stage. Pure chemical substrates (e.g. H2, CO2, acetate) may also be used to feed in the methanation stage.
Hello Alam surya, Thank you for the answer .But I am looking for alternative sources rather than the pure chemical substrate.Can you suggest me any article or research related to this topic?
Im not sure what do you mean with "alternative sources" because it is not clear "alternative to what". I suggest you start whith what you have nearby: cattle manure, food waste, ...? Or if you can clarify your question maybe we can suggest acordingly.
Prudhvi, yes it is possible. To get the 1-2-3 know-how, check my articles dealing with
1) two stage anaerobic digestion and 2) inhibition of biogas production
Sections: Materials and methods
Kind regards
Liliana Marquez-Benavides Alternative Sources to produce the substrate in the lab for methanogenesis step without hydrolysis step in order to start producing methane instantly without waiting for the entire hydrolysis procedure to complete and to get the products from hydrolysis that can be used by methane forming microbes as a substrate during methanogenesis.
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