You can use 1% agarose in barbitone buffer pH 8.6.

Read page 85 and 86 in following link:

https://books.google.com/books?id=vnR6MqBAueIC&pg=PA86&lpg=PA86&dq=serum+protein+electrophoresis+harold+varley,+practical+clinical+biochemistry&source=bl&ots=mgmV3VTED_&sig=ACfU3U1e36xxUBdNXjz0D7LJr4FpzI6FUg&hl=en&sa=X&ved=2ahUKEwiK3v2gqvLnAhU6HzQIHYeBCLkQ6AEwA3oECAsQAQ#v=onepage&q=serum%20protein%20electrophoresis%20harold%20varley%2C%20practical%20clinical%20biochemistry&f=false

Please look at the following below links which may help you in your analysis:

https://www.interchim.fr/ft/4/47255g.pdf

https://www.helena.com/Procedures/Pro001Rev6.pdf

http://dmbj.org.rs/jmb/pdf/2010-1/2.pdf

Thanks

Thank you everyone, you helped me a lot.