Agarose amount in serum protein electrophoresis?

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Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

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Bhairavi Vajaria

You can use 1% agarose in barbitone buffer pH 8.6.

Read page 85 and 86 in following link:

https://books.google.com/books?id=vnR6MqBAueIC&pg=PA86&lpg=PA86&dq=serum+protein+electrophoresis+harold+varley,+practical+clinical+biochemistry&source=bl&ots=mgmV3VTED_&sig=ACfU3U1e36xxUBdNXjz0D7LJr4FpzI6FUg&hl=en&sa=X&ved=2ahUKEwiK3v2gqvLnAhU6HzQIHYeBCLkQ6AEwA3oECAsQAQ#v=onepage&q=serum%20protein%20electrophoresis%20harold%20varley%2C%20practical%20clinical%20biochemistry&f=false

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.interchim.fr/ft/4/47255g.pdf

https://www.helena.com/Procedures/Pro001Rev6.pdf

http://dmbj.org.rs/jmb/pdf/2010-1/2.pdf

Thanks