In what ways the stray dog testicles (after castration) are useful in research purposes. Now having fresh 50 pair of testicles & 50 whole ovary with full length tract preserved in 10% Formalin.
Research can consider histomorphometric studies of the testicles, having in mind age at castrartion. Also developmental studies can be done considering the anatomical changes with age. Another aspect could be to do a biochemical components with respect to cell biology
Stray dog testicles could be also used for many molecular-epidemiology and histopathology studies. You may find valuable data about vectorial (eg. tick-borne) and zoonotic diseases, eventually biomarkers for environmental polution
1. cumulative chemicals residues like lead, cadmium, pesticides etc
2. histological lesions suggesting exposure to teratogenic/mutagenic chemicals or cumulative toxics
It is important to preserve the testes properly for future studies. Small pieces should be dissected and stored quickly in preservative so the tissue is fixed as quickly as possible. Each testis should be stored in a separate container to avoid cross-contamination. The containers should be clean and sterile if possible. A record of the size, weight and condition of each dog should accompany the specimens. One could evaluate if there are seasonal differences, if apparent age has an effect, if the tubules differ in size or characteristics, etc. If you also recover the epididymis, it can be studied and the number of sperm in the distal segment quantified. The DNA might be useful for those studying migration of dog genes across regions.
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