I am interested in measuring the thickness of flatworms in whole mounts. I am considering using the micrometric knob of the microscope calibrated against a know thickness. Has someone any idea or experience to share?
Thanks in advance.
Unfortunately, I've no experience of measuring the thickness of worms in this way.
Your method should be relatively easy if your microscope is equipped with a motorized stage. Else I don't see how you will know exactly where you are, unless you have a finely graduated meter on the stage. Mitutoyo sell products to measure thin objects. They have microscopes especially designed for that or, if you don't want to pay for a miicroscope (I don't know how much they sell), a micrometer gauge could do the trick (although less precise, and you'll have to subtract the slide and coverslip thicknesses).
Miscrosopes: https://www.mitutoyo.co.jp/eng/products/gazoukogaku/kenbikyo.html
Miccrometers: https://www.mitutoyo.co.jp/eng/products/micrometer/sotogawa.html
Yes , You can measure thickness of flateworm by using camera Lucida diagram.
Should make a plasticine model (take fixed amount of it - eg 10 ml) and calculate the coefficient of form. After that recalculate for actual length! Look Nesterenko & Kovalchuk paper (Acta Hydr. Hydrobiol) in my RG site.
Try - Andrey
Juan. Presumably, you refer to existing whole-mounts (likely squashed already), which cannot be unmounted and sectioned. Perhaps you could look at laserscan or other 3D microscopes which can re-assemble images at 90 degrees or 3D software, e.g. http://dx.doi.org/10.1371/journal.pone.0077650
David
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