Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

How to get rid of Matrigel during enteroid passaging?

Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...

10 March 2020 1,805 1 View

Is there a way to edit the citations in MENDELEY so it's in the order of citation in the text body instead of Author?

Does anyone know how to change the citation style in Mendely in order to sort the references according to the order of citation in the paper? So that in the text is displayed only the number (eg....

01 January 2020 746 2 View

How to avoid impurities in immunoprecipitation with magnetic beads?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

09 September 2019 6,947 4 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

What are these spheres created by genetically modified Ralstonia eutropha after fermentation of hemp?

Hello microbiology researchers working with Ralstonia eutropha. The organism we have created contains two genes that allow R. eutropha to ferment hemp and produce P4HB. When we began the culture...

13 November 2018 3,838 3 View

Which are the incubation conditions for a biotinylated antibody with a streptavidin coating?

I have to do a sandwich coating with: 1) biotinylated BSA 2) streptavidine 3) biotinylated antibody Which are the common conditions for the incubation regarding each step? Buffer type and pH,...

08 November 2018 770 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

How can I measure training as a moderator?

I am working on technology adoption. I want to explore the moderating role of training on the relationship between the factors and the intention to adopt the technology. So, how can training be...

31 January 2021 3,755 3 View

Tissue Dissociators for mouse spleens and lymph nodes?

18 January 2021 6,804 6 View

What are examples of data-intensive research leading to totally new insights?

Data-intensive research is still all the rage these days. Breakthroughs in AI (and especially NLP) seem likely to only accelerate its adoption. But can anybody provide examples (preferably from...

17 January 2021 10,008 4 View

Servers for finding free energy and dissociation constant of protein-protein docked complex?

Hello everyone, I would like to get help about finding any server or application which can give me info about free energy and dissociation constant of protein-protein docking outputs. I have...

15 January 2021 587 3 View

Can anyone provide articles related to TAM model adopted with the mobile payment or technology adoption ?

Hi all Can anyone give me some research papers which have shown behavioral a behvaioural intention leads to actual uses i.e. adopted full TAM model in the context of mobile wallet, mobile...

30 December 2020 7,221 4 View