Hi, I have added missing residue in PDB id: 5ZWJ by using Charmm-gui (PDB Reader & Manipulator) but in added PDB, I am getting all HIS protonated to HDS. One other problem I found that in last residue 1028, there is presence of OT1 and OT2 (image attached).
Can anyone give some solution or suggestion for adding missing residue?