01 January 1970 1 10K Report

I'm trying to find the Activity Budget of chimpanzee in the wild based on the diurnal activity ( ≃ 13h), shown with percentages. I need it for comparing with captive chimpanzee's activity budget. Thanks to those who will contribute.

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Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

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Which negative control do you recommend for In vitro Foam Cell Formation Assay?

Hi everyone I have to perform the Foam Cell Assay with OIL RED O staining. Can you recommend a compound that inhibits LDL oxidation? Also, can anyone recommend oxLDL to use?

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Can I transfect differentiated cells?

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Research article about minors community?

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What do you think I'm doing wrong?

Hi everyone, I differentiate THP-1 in macrophages with PMA 48h + 24h rest. After treatment with LPS I would expect an increase in ROS. But this increase compared to control I cannot get. I have...

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Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

20 March 2024 5,496 4 View

When does the Griess colorimetric assay produce yellow instead of pink?

I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...

18 February 2024 3,338 0 View

Hello there, any suggestion to calculate equilibrium constants without NMR variable T?

If we have an host-guest complex, in water an equilibrium occurs. So you can have ComplexGuest + H2O ComplexFree + GuestFree. So I can calculate equilibrium constant, but in the most of...

31 January 2024 335 1 View

What medium can I use (instead of RPMI) on differentiated THP1 if my goal is to measure small changes in nitrate levels?

I differentiated THP1 cells into macrophages. Subsequently, macrophages will be treated with substances. Next, the Griess Reagent test will be used to determine nitrate levels. The Griess kit...

24 January 2024 9,551 1 View

Way of conversion of gauge pressure for thesis?

In my pressure vessel, I filled half with DI water. Then reset my pressure sensor to 0 at room temperature. After, I heated the vessel to increase the temperature. It increased the pressure in the...

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