What is the smallest Archaeological feature size that has been successfully analyzed using either LIDAR or Satellite Image analysis?

I am considering the viability of using AI in conjunction with either LIDAR based Digital Elevation Models or Object based image analysis of Satellite Imagery to predictively locate Inuit...

25 October 2020 9,525 2 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How do anthropogenic pressures influence and will influence coastal groundwater according to the climate and its evolution?

Groundwater is of utmost importance in Haiti. It is the main source of drinking water for the Haitian population. The degradation of the quality of this resource is a major issue in the context...

29 June 2020 8,626 6 View

No title

Whether for a dissertation, a thesis or any other research work, there will be a problem in which it is clearly posed what is causing the problem and which imposes on the researcher the need to...

29 June 2020 4,902 9 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

What would be standard formula/equation to derive post-test mean & standard deviation values from the established study for meta-analysis?

Since in some studies, authors only report the mean difference and mean difference standard deviation. Kindly, help me out to get the absolute mean and SD of post-test measures. Thanks

22 March 2020 5,358 7 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

Why cell proliferation increased after change amino acid by SDM?

Dear Scientists, I am working with a gene of interest in colorectal cancer, which has no role in cell proliferation. In addition, by knockdown this gene, the FAK and SRC protein expression are...

09 October 2019 6,257 3 View

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. Any idea?

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. I found out that at higher CEA content, particle size is...

03 July 2019 4,764 4 View

Does anybody know a company specialized in platinum machining?

Good day! Does anybody know a company providing on-demand platinum machining service? Thank you in advance for your help! Kind regards, Reda

14 February 2019 9,275 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How can I analyze the morphology of microglia (ramification, braches, soma, etc.) via Fiji/ImageJ?

Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...

31 January 2021 3,855 3 View

How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

How to make MRI image segmentation and create binary mask with imagej?

I have a number of MRI Dicom images. I need to create a binary mask with ROI for feature extraction. How I can do this by imagej?

25 December 2020 8,396 2 View