hi i am applying mechanical force on my cells and would like to know if you have any tools available for looking at actin stress fiber length, thickness etc.
imageJ plugins eg.
I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...
19 June 2024 1,612 8 View
Which is more polluting to the environment, extracting crude oil and its derivatives or extracting lithium, and how to get rid of it?
14 May 2024 7,181 2 View
Are the licensing rounds that China recently obtained from Iraq considered a victory for the Chinese pole over the American pole?
14 May 2024 3,151 2 View
I am planning to start a study utilizing 810 nm light to stimulate the vagus nerve bilaterally and am looking at exclusion criteria. Thank you.
26 March 2024 9,614 3 View
variations in belief, traditions within and across culture in SPED
24 March 2024 2,551 0 View
I design ELECTRO_OPTIC MODULATOR it using FDTD LUMERICAL that contains 3 waveguide. The intermediate waveguide is a plasmonic that contains SI, ITO, HFO2, and AU. I need to add a voltage to the...
28 January 2024 8,773 2 View
What program is used to draw here? Please explain
28 January 2024 6,484 0 View
Do these celebrations contribute to promoting economic growth, employment and increasing tax revenues in general?
30 December 2023 2,049 1 View
What is the cost of New Year celebrations for major cities globally and what is its impact on the global economy?
30 December 2023 1,175 1 View
What is the total financial cost of these celebrations, including the costs of fireworks, artistic appearances and concerts?
30 December 2023 4,793 0 View
I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...
23 July 2024 4,461 1 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
11 July 2024 1,464 7 View
Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...
01 July 2024 8,964 1 View
Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...
12 June 2024 7,332 0 View
I want to analyze some images (nearly 1000) in a loop. I want to analyze HSV and RGB. I have masked those images in ImageJ, which is binary masked. I tried to explore them in R, but all the...
04 June 2024 5,098 1 View
I have taken fluorescence images of the control and treated sample(Immunofluorescence, tissue sample) at the same settings. So I need to measure the change in fluorescence intensity of the treated...
16 April 2024 2,093 1 View
We have a demarcation and analysis protocol in ImageJ for images of C. elegans stained with Oil Red O. However, it does not seem to be the best way and we were unable to find an easy-to-execute...
15 April 2024 2,031 1 View
Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...
11 April 2024 4,457 1 View
How to count multilocular adipocytes on a slide with a white adipose tissue sample stained with H&E? I had done the count on the entire slide, but the articles put it in % of cells or...
10 April 2024 3,997 2 View