For my study, i want to ascertain whether or not E. Coli O157:H7 is the predominant serotype of EHEC in West Africa, or Africa as a whole.
Materials science journals
09 July 2020 4,774 5 View
Dear researchers, I would like to hear and learn from your suggestions based on your general experience, during the course of your doctoral thesis writing.
20 May 2019 1,637 14 View
I prepared serial dilutions from a food sample and plated 1 millilitre of a dilution on VRBGA. After incubation for 20 hours i realised the agar had turned yellow. What could be the cause of this...
13 February 2019 1,056 2 View
I am writing a research proposal and proposes to run a simple linear regression on impact of participation (independent variable) in social policy on well-being (dependent variable) of older people.
05 June 2017 1,324 3 View
Both involve the loss of muscle coordination and balance. But how do the symptoms of cerebellar ataxia differ from that of cerebral palsy?
18 July 2016 9,166 8 View
The Genetic Algorithm can be modified in different ways. Even Goldberg's book, Genetic Algorithms in Search, Optimization and Machine Learning, specifies various enhancements that could be done...
11 April 2016 5,289 2 View
I know that a large proportion of the muscles in the body are active for a person during her/his gait. But I'm not sure exactly (or approximately) the value of the proportion in percentages with a...
09 March 2016 531 9 View
Generally, a machine learning system would only make a single prediction among available classes (classification) or predict a single real valued number (regression) based on the given set of...
28 January 2016 5,321 6 View
Apart from their apparent difference -- the area of applications differ -- what may be the precise differences between clinical and general gait analysis? To which degree is one associated with...
27 October 2015 2,292 9 View
The inventors of the AES Rijndael state that there are no 'linear' mathematical relations between plaintext and the corresponding ciphertext when passed through Rijndael. How can you prove this...
30 May 2014 9,389 2 View
i would to know some of the research gaps in the artificial intelligence field in most african countries.
03 March 2021 6,145 3 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View