Absorbance of hydrlolysed Nitrocefin substrate by high concentrations of B-lactamase?

19 July 2023 0 7K Report

Why tha absorbance values of hydrolysed nitrocefin by means of high concentraations of B-lactamase (micromolar scale) reducing by the time when assayed in microplate redaer on 490 nm in contrast to nano molar scale?

Please see the attached graph.

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