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Questions related from Ammar Ibrahim
In many litratures, I found the allosteric site in TEM-1 is H10 helix , but the explination of how this allosteric site affected by the inhibitors sounds mysterious to me. Can you please...
06 October 2023 5,491 1 View
Is there any esay way to clean quartz uv cuvetts from hard sticked precipitites using availble chemicals in the lab such as acids or basis, not expensive commercial products ?
30 August 2023 7,803 1 View
In order to perform Michaelis-Menten plot to calculate Km for oxa beta lactamase , I used nitrocefin as substrate , 100mM sodium phosphate di basic and 25mM sodium carbonate as buffer pH 7.3...
15 August 2023 2,516 9 View
Why tha absorbance values of hydrolysed nitrocefin by means of high concentraations of B-lactamase (micromolar scale) reducing by the time when assayed in microplate redaer on 490 nm in contrast...
19 July 2023 6,507 0 View
I would like to order clavulanate for my assay inhibition of B-Lactamase, but I found many types of them such as Vetranal, Impurity A , Impurity G , etc How can I decide What is the most...
27 June 2023 771 0 View
What is the concentration of Clavulanate inhibitor that I should use in assay to obtain full inhibition of B-lactamase activity?
16 June 2023 2,779 1 View
I am trying to find some effective epitopes on the protein IL13R alpha 2 . What a method that I should follow ?
12 June 2023 7,307 1 View
I need help with O-Phthaladehyde (OPA) Fluorescent Peptide Assay. I am looking for the proocole or the procedure .
02 June 2023 1,902 1 View
What is the hydrolysis buffer? After running hydrolysed Nitrocefien im kinetic mode , how can I draw the standard curve ? I mean at what time set I plot the absorbances versus the Nitrocefine...
21 May 2023 4,453 10 View
Is there any way or calcations to convert the particle size of nanoparticle to molecular weight KDa?
01 January 1970 1,973 4 View
Regarding AR2G sensors, If i need to do kinetic assay for binding between analyte molecule (such as nanoparticles have free NH2 and NH groups) and protein, (the nanoparticles are larger than the...
01 January 1970 8,218 2 View
