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Is folic acid cytotoxic to human kidney cells ?

My group is interested in folic acid cytotoxicity in human kidney cells. Are there any reports available showing toxic effects of FOLIC ACID as in the case of mice kidneys? Any insights will be...

19 October 2020 2,273 3 View

How to generate a Force which has a duration of 0.3 ms and allows excitation of waves of frequencies up to 80 kHz in matlab.?

Actually this is the impulse force, which is to be applied on the rod element?

09 September 2020 8,080 3 View

How to calculate Abaqus software compatible material properties from the data described below?

From testing data: yield stress, ultimate stress, total strain, ratio of strain at elastic limit by strain at strain hardening and the ratio of elastic yield modulus to the strain hardening...

06 September 2020 5,279 10 View

How to find the flame speed from fluent??

I had the simulation upon combustion in ANSYS FLUENT. Reaction was based on the "Federick and Dryer" global mechanism. Solution was able to obtain convergence but i need to find the flame...

18 June 2020 1,614 6 View

What is a popular book for understanding laser fundamentals and evolution and types of lasers?

I looking for books, channels, videos and PDFs regarding laser and particle interaction and evolution of laser power and laser types and how laser makes the physical changes on the ceramic...

05 April 2020 3,396 25 View

How to determine the number of clusters in Cluster Analysis using SPSS?

I am trying to analyse students data to determine the common characteristics of mathematics students. Cluster Analysis would be valuable to get these common characteristics. Any idea or any...

27 March 2020 2,949 5 View

I would like to know if there is anyone who is working on CEP126 gene(also known as KIAA1377)??

As there is very little data available for CEP126 gene on databases, i would like to know if there is anybody or any lab working on this gene.

31 January 2020 1,860 1 View

How Can I get the datasets for in-vehicle network CAN bus security research ?

I would like to study the security in in-vehicle network CAN bus. Denial of service (DoS) and Replay attacks would be launched and ways to solve these problems is to be studied. Machine learning...

26 January 2020 3,061 3 View

Calibrating stereoscopic system with a laser line projector and camera?

I want to set up a framework for combining a laser line projector and a camera to extract the 3D coordinates of a target object. I cannot find much literature regarding this using active light...

16 October 2019 3,582 1 View

Can anyone explain the unexpected protein concentration loss in buffer exchange?

I have a stx2 236 TALE-A protein in glycerol stock at -20C. It's concentration is 3.2uM. I did buffer exchange with HEPES buffer 3 times (100uL protein+ 300uL of buffer each time) in a micro...

05 September 2019 4,847 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View