Does Arsenophonus replace the other gut bacteria in insects?

02 November 2020 4,628 4 View

In terms of camera based fluorescence microscopy, what does time interval mean?

I am getting slightly confused here... When the term 'time interval or delta t' is used, does it explicitly imply time between successive frames or can it also refer to the time taken to capture...

19 October 2020 3,105 5 View

Does fluorescence based imaging improve spatial resolution?

I understand specific techniques based on fluorescence and other methods can enhance the spatial resolution beyond the diffraction limit of lambda/2NA (xy-plane) in what we call as the...

03 September 2020 8,786 3 View

Why my RAPD primer for fungal species is not working properly?

hello everyone, I m trying to perform RAPD-PCR for fungal species. till date total 40+ primer i have tested but no positive result i have got, only one primer showing reproducible band again and...

27 February 2020 8,785 3 View

Is it compulsory to perform the curve fitting technique during the interpretation of subsurface resistivity data ?

I have 1-D subsurface resistivity measurements ( using Schlumberger electrode array) as well as borehole data up to the depth of 30 m. Is it compulsory to perform curve fitting using the master...

16 January 2020 5,517 6 View

How to quantify Likert Scale (5 or 7 point)?

I am interested to know the reliability of quantifying primary data collected by using Likert Scale. Can we measure gaps in the variables? How to perform in SPSS?

15 January 2020 8,159 5 View

Is citation count a good metrics to measure quality of a researcher?

Citation count tells that a particular article is important to discuss. It does not tell that the article is criticized or appraised.

09 January 2020 3,120 6 View

Anyone knows about mann-Kendall test for long term rainfall and temperature data. How to run mann-kendall test using R-software?

please explain with example for understanding of new person.

19 September 2019 9,594 5 View

What are things to remember while designing primers to amplify cDNA corresponding to a specific transcript isoform?

I want to amplify cDNA for a specific RNA in plasmid, I will transfect it into mammalian cells, generating full length transcript(3kb) is necessary for my experiment.

05 May 2019 5,290 6 View

How to analysis a categorical data set, in which independent and dependent variables are categorical?

I have a dataset having 56 variables, in which 4 dependent and 52 independent variables. In those independent variables 45 variables are categorical and 3 dependent variables are categorical...

02 May 2019 2,253 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View