A software for sequential protein digestion?

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Transfection in HEK293T cells?

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How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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EDC carbodiimide and NHS easter 's half life among different pH?

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Does post-translational protein modification cause devisions on observed pI verses calculated pI?

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Sandeep Pandey

PeptideMass can be used to cleaves a protein sequence from the protein database or can be entered the protein sequence manually with a chosen part and computes the masses of the generated peptides.

Hiba Salim

Thank you for responding, but I mean doing two digestion steps with two different enzymes. Sandeep Pandey

Abeeb Abiodun Yekeen

Hi Hiba Salim ,

You may check out Expasy's PeptideCutter tool, or the more recently published Rapid Peptides Generator (RPG)...