How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can children from 1 year old to 15 years be given MODANA and Pfizer Anti-COVID-19 vaccine?

The clinical disease of COVID-19 has been low in children generally and vaccine has been develop.Any evidence of its safety,efficacy and risk for allergy in children? any exemption for children...

23 December 2020 7,177 12 View

Can i run native agarose gel electrophoresis in TAE buffer?

I am characterizing different snake venoms. As snake venom is a mixture of various large protein complexes (with both acidic and basic proteins) i want to run native agarose gel electrophoresis...

07 December 2020 4,629 3 View

How to dye the spider gland of a spider to see in fluorescence (488nm)?

I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to...

14 October 2020 3,986 10 View

Is there anyone who can help me to learn Venom Proteomics?

I am interested to do my PhD research on venom proteomics. I don't have details idea of it. I am a beginner in this area. Is there anyone who can support me to provide necessary information...

03 August 2020 1,820 3 View

How can I detect the presence of antibodies in plasma using electrophoresis?

Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...

30 July 2020 3,400 1 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

Dataset on Allergies?

Currently Im working on my thesis in PhD. Unfortunately I am not able to find dataset on seasonal allergies. Kindly guide

08 September 2019 346 3 View

Is there anyone who has worked on Scorpion venom or remained related to this work?

I am a Student of BS zoology 7th semester and i am much interested in doing work over scorpions in any aspect which is still to be identified. If there is someone please guide me Thanks

08 September 2019 7,118 5 View

Dataset on Allergies?

Currently Im working on my thesis in PhD. Unfortunately I am not able to find dataset on seasonal allergies. Kindly guide

31 August 2019 1,802 3 View

What solution for allergy in babies ?

Hypoallergenic milks used to limit the risk of allergies in children are increasingly controversial. Would not the solution of the future to fight against allergy be the induction of...

07 August 2019 4,278 4 View