How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

A three level hierarchical data structure in multilevel modelling, for multiple response question?

Im refering to Andy Field's example in his book Discovering statistics using SPSS, 3rd edition, page 728, where he gives an example of a data structure with level 1 variable is a memory recall...

26 December 2020 9,717 6 View

What is the reasons of increased th molecular weight of some chemicals in plant when the concentration of a pollutant that effectin on plant?

please need paper explain this case

23 December 2020 2,657 2 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

How to measure internal validation of multiple regression using a split-sample method with dichotomous variables?

I would like to validate a regression model using a 50-50 (or 80-20) split method. In my initial multiple regression I have entered all predictor variables using 'enter' technique. My sample size...

20 October 2020 2,849 5 View

How to aggregate expert opinion for Fuzzy AHP methodology?

Any recommended method or approach? Appreciate the support.

11 October 2020 5,290 6 View

How is solubility of CO2 or air in organic solvents such as xylene or cyclohexane?

For some reason, I need to inject a dispersion of an organic solvent and organoclay into a block of soil sample. the organic solvent probably is xylene.

29 September 2020 6,348 4 View

What are the psychological effects on children during the COVID 19 pandemic?

I am working on the Psychological impact of COVID 19 on children of health care workers. i need references and guidelines for research on this topic.

20 July 2020 2,791 19 View

I need a protocol for Cas9 expression in E coli?

Hello Dear researchers, I am trying to express Cas9 vector in E coli but finding difficulties. After IPTG induction I am unable to get any protein(Cas9) via His-tag chromatography. Also, there is...

17 June 2020 1,170 2 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View

What assays / application would benefit the most from absolute quantitative fluorescence measurement ?

Many assays, including Cell-based assays, QPCR, some ELISA test, rely on the presence/absence of fluorescence. Diagnosis applications about cancer or antiobiotic resistance uses fluorescence ratio...

21 October 2020 1,159 3 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Asides using ethidium bromide, what other methods do we use for plasmid curing?

Is there any other substance, either radioactive or not, that can be used for plasmid curing? And what other methods?

23 August 2020 5,254 3 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View