95% ethanol for DNA extraction using Qiagen DNeasy Blood and Tissue Kit?

19 August 2021 1 3K Report

Hi All, I am using the a Qiagen DNeasy blood and tissue kit for the extraction of DNA from human blood sample and it recommends using 96-100% ethanol in the protocol. Our lab only has access to 95% ethanol, so I was wondering does anyone use 95% ethanol with these kits and do the extraction results differ with 95% compared to 96-100%.

Thanks!

Hanna Alalam

I have used 95% with these kind of kits and the results were fine (could do all the downstream DNA manipulation/transformation/sequencing). In certain kits like zymo kits they actually have an adjusted volume for 95% vs 96%-100% ethanol. I never compared the yield between the two different concentration.

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