Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Is it possible to re-use a MK801 20uM solution when used as a bath for fish?

We are exposing zebrafish to an MK801 solution and we are thinking if it is possible to re-use the solution (between 3 and 5 times in a row) in order to decrease the amount of waste produced,...

11 October 2020 7,391 1 View

CRISPR-Cas9: Are there any alternatives to the use of fluorescent PCR and capillary electrophoresis?

We are trying to find alternatives to the use of fluorescent tagged primers and capillary electrophoresis to access somatic activity of the target gene. Thank you!

25 September 2020 4,761 3 View

How can I do a soil characterization using TGA analysis?

11 August 2020 1,863 3 View

How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

How can i optimize the amount of protein obtained in tissue homogenates?

How can i optimize the protein amount obtained in cardiac homogenates when working with limited amounts of tissue (mice)? (Homogenates will be used to measure oxidative stress biomarkers)

05 May 2020 1,715 8 View

Protocol: Single-Locus and Multilocus DNA Fingerpriting, Bruford et al. 1992?

Hi everyone! I know this probably isn't the right place for this, but I have exhausted all other options to access this paper. Does anyone has a pdf copy of the chapter "Single-Locus and...

25 March 2020 2,540 5 View

Is there any protocol capable of simulating an epileptic seizure in vitro using direct electric stimulation?

I am trying to simulate epileptic seizure activity in cell cultures by using direct electric stimulation. However, I am struglling to find an acessible and relatively easy-to-use protocol. Thank...

28 January 2020 4,951 2 View

Statistics - Multiple Regression Model. What's the equation that better descibes the relation between Mass and residuals?

I'm doing a statistic test (statistic exercise) given by my statistics teacher. The goal is to study the possible relation between the mass and wetfrass (residuals) produce by a certain...

11 January 2020 3,853 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View