2D rehydration buffer for zymography of plant amylase & protease?

More Prafull Gadge's questions See All

Could we use atomic force microscopy to measure adhesion strength of hard thin coatings on Tungsten carbide? Where do i avail this facility?

Hello, I need to check adhesion strength of hard thin coating on Tungsten Carbide substrate by scratch test which is quantitative. Thickness of coating is 5 microns.

01 June 2021 9,529 4 View

Transient expression of a gene by using just the retroviral transfer plasmid possible?

If I transfect my cells just with the retroviral transfer plasmid ( pWZL- Hygro) without the packaging vector (pCl-eco), would it only be transiently expressed? The retroviral vector sequence...

12 February 2020 8,626 3 View

Which is the best detergent for studying protein interaction by co-IP experiments?

I am interested in studying the interaction of outer mitochondrial membrane bound proteins by co-immunoprecipitation. Which would be the most suitable detergent for this study: Digitonin Vs CHAPS...

19 August 2016 1,738 3 View

Are there Prestained Marker available for Native Gel to enable monitoring during electrophoresis?

Hi Everyone I am doing Native Gel electrophoresis for quite some time and have been using own made protein standards to get an idea about the size of my target proteins. These markers are...

31 March 2016 5,391 3 View

What is the difference between absorption and Excitation spectra?

I am performing cellular uptake studies with peptides labelled with fluorochromes. I did flow cytometry experiments and now I want to validate it by fluorimetry by measuring the emission spectrum...

11 March 2014 9,276 4 View

Does anyone have experience with competition assay with labeled and unlabeled peptide?

I am working on development of cell type specific delivery peptides. To prove the receptor specificity of these peptides, I am performing competition assay between Fluorochrome labelled and...

07 February 2014 5,893 8 View

Any insight as to Flow Cytometry vs Fluorescence Microscopy?

I am performing experiments for cellular binding of specific peptides. These peptides are labelled with FITC. For some peptides, I do not see any fluorescence in the microscope, however and flow...

04 February 2014 2,558 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,559 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

What binder would be the best choice to modify glassy carbon electrode with a 2D Material like Graphene or MXene?

I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?

23 July 2024 5,776 2 View

Domancar Orona-Tamayo

Remember, chaotropic chemicals denaturing your proteins, so, you need set your buffer and probe, that is the "trick" in zymoproteomics, I think you need a "deep look" these papers:

http://www.sciencedirect.com/science/article/pii/S0308814610007260

http://www.sciencedirect.com/science/article/pii/S0176161711001556

http://www.sciencedirect.com/science/article/pii/S0308814611008223

Cheers!!!

Prafull Gadge

Thanks Domancar. I found this information is valuable...