My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

How to prove the formation of a synthesis product using NMR ?

Hello, I am trying to identify the formation of a synthesis product. Knowing the precursors, I focused on the identification of the H-C long-range coupling between sites (a/b) and (a/c) using...

19 January 2021 8,438 5 View

Is there any default ''TopSpin'' [H-X] HSQC pulse program ?

I would like to conduct a [1H-13C]- HSQC (optimized for one bond H-C connection) experiment. What would be the best default topspin pulse program and parameters set to use ? Thank you.

14 December 2020 6,956 5 View

How can we quantitatively assess the value of an educational program (EP) ?

The question can be understood in terms of a secondary and auxiliary question, I.e. how we can say that EP1 has a greater intrinsic value as compared to EP2 or Vice versa ?

06 December 2020 916 5 View

Why does the command ''Topshim'' fails ?

Hello, I'am trying to run the 1H NMR spectrum of an ionic solution (Bromide Salt). the solution is too viscous so I had to randomly add a certain volume of deuterated chloroform, and then...

30 November 2020 8,569 1 View

How can I improve the Cronbach Alpha results without deleting too much items?

I am running pilot test and the Cronbach's alpha is 0.71 for all of the items. However, when I come to analyze every section of the questionnaire individually, In found that it is too low. And I...

10 October 2020 6,213 1 View

How to interpret DSC heat flow curves?

I'm running DSC for a mixture of 2 polymers and I need help to interpret the heat flow curves

13 August 2020 2,993 4 View

Someone from Alexandria University his name Mohammed Salem claimed three of my papers to him in research gate, how can this problem be resolved?

Mohammad Salem from the Alexandria University claimed the authorship these three papers: 1. First Report of Fusarium verticilloides causing Banana Fruit Rot in Jordan 2. First report of cucurbit...

26 July 2020 9,647 3 View

What is the best method to analyse survey data in auditing research ?

After the field data has been collated using Likert-style instrument e.g 'Strongly agree,Agree,Neutral to Strongly disagree, , what is the appropriate test statistic to use ? (i.e easy to...

18 May 2020 5,039 4 View

Can Neural Network predict an output value?

If I have a set of input data and a set of output data as shown in attached file, Can a Neural Network predict an output if the input data out of the given input data? Example: if the new...

11 June 2019 2,899 9 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

What makes yellow color appear in phosphorus sample after autoclave digestion?

I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I...

25 January 2021 206 3 View

Disposing Congo Red Agar?

Hi all, Hope you are well. My lab is planning to use some Congo red agar (0.01%) for Shigella flexneri, and we are wondering how do you dispose of the used agar plates. The Shigella contaminated...

13 January 2021 9,823 2 View

How can Sea water impact the hydrodynamic size of Nanoparticles differently than the same Sea water if it is autoclaved?

I am using the same sea water as autoclaved as well as non-autoclaved after adding the nanoparticle. I am getting different Hydrodynamic size for the same nanoparticle.

06 January 2021 3,184 3 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

How to get dry petri dishes in glass after wet sterilization?

Hi, I warping my plates with paper, later put them in the autoclave (use wet sterilization cycle) but still some plates are get wet after ends the cycle. How to get dry the petri dishes? and if...

13 December 2020 8,062 4 View