If I analyze bacterial population of a sample (soil) by 16s rDNA targeted Next Generation Sequencing (from community /environmental DNA ) then the method should be called as Metagenomics or Metabarcoding?
No, it is not metagenomics. In the same way that you don't do genomics of an organism by studying a single gene. Think about it, you don't call genomics when you use 16S to identify a strain or describing a new species.
The Genomics Standards Consortium even manage the Minimum identification about X sequences with different standards, MIMS and MIMARKS for metagenomes and marker gene sequence studies respectively.
Appropriate terms would be 16S rRNA gene survey, amplicon sequencing, metabarcoding or even metagenetics.
Some opinion about:https://phylogenomics.blogspot.com.au/2012/08/referring-to-16s-surveys-as.html
http://www.opiniomics.org/youre-probably-not-doing-metagenomics/
Hi Shan,
Definitely, Metagenomics, because you start from meta -samples (combination of the different population) and then sequencing and analysis of genomic information so it is a Meta + Genomics, while metabarcoding is differentiated a sample by another by barcodes only.
So, better to use the term Metagenomics rather Metabarcoding.
Hi Shan,
While I do agree with Abhijit, that you are analyzing meta samples and looking at genomic information, it would be insufficient to say it is metagenomics, as this is the term used for shotgun environmental sequencing. Metagemonics looks at the entire genomic complement of an environment, not just one gene.
The correct terminology would rather be Marker Gene Metagenomics, or Marker Gene Analysis. And it can most definitely also be referred to just as 16S rDNA analysis.
Hope this helps.
No, it is not metagenomics. In the same way that you don't do genomics of an organism by studying a single gene. Think about it, you don't call genomics when you use 16S to identify a strain or describing a new species.
The Genomics Standards Consortium even manage the Minimum identification about X sequences with different standards, MIMS and MIMARKS for metagenomes and marker gene sequence studies respectively.
Appropriate terms would be 16S rRNA gene survey, amplicon sequencing, metabarcoding or even metagenetics.
Some opinion about:https://phylogenomics.blogspot.com.au/2012/08/referring-to-16s-surveys-as.html
http://www.opiniomics.org/youre-probably-not-doing-metagenomics/
Dear Abhijit Maji, Andries Johannes Van der Walt and Xabier Vázquez Campos, thank you for sharing information about the concept.
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