How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can "Natural Language Processing" be used for modelling information flow between platforms?

Dear fellows, I am working on my bachelor thesis right now. My vision was to extract some sort of information flow between platforms. Concretely, I wanted to analyse 4chan /pol/ posts from 2016...

05 January 2021 9,424 3 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

Can i know the conversion of human SAR value to that of a mice?

The phone have 'Head SAR' of 0,527 W/kg, and I give exposure to male Mus Musculus 3G (2100 MHz) and 4G (1800MHz) each group for 1 hour per day for 30 day. Is the result will be the same in human?...

26 December 2020 2,475 3 View

How to calculate the flow rate required for fluidization for a fluidized bed gasifier ?

We need to calculate the flow rate of air required for fluidization of the bed. We have a compressor which gives air at 7 bar and a 1 bar rotameter for measuring the flow rate in LPM. How can I...

16 December 2020 2,092 3 View

Can Apparent Activation Energy determine Conversion Rate in a catalysis reaction?

Recently, I got a concept that is contrary to my understanding from two papers. It can be described as follows, in brief: There are two catalysts for the same reaction, on the catalyst with higher...

09 December 2020 2,406 28 View

How to convert Unit/mL enzyme concentration to FPU/mL or FPU/g concentration?

Is there any conversion formula for this purpose.

06 December 2020 3,283 3 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View