Review and cite AGRONOMY protocol, troubleshooting and other methodology information | Contact experts in AGRONOMY to get answers
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Questions related to Agronomy
Hi, I am trying to purify alpha synuclein protein from E.coli BL21(DE3) cells. The plasmid is pET21a alpha-synuclein. Can anyone help me with the protocol. Thank you
16 January 2024 8,756 2 View
I need to collect plant samples (stems, sores, etc.) to later be scanned in a micro tomograph, and I would like to know if glycerol is a good idea for a preservation medium, or can it generate...
09 January 2024 2,799 1 View
I have an unknown concentation source of methane. With a hole the methane is diffusing to the air. the air methane concentration just outside the lekage is 120 ppm. Can I assume the methane...
18 December 2023 5,843 1 View
I want to know more about Au deposits.
17 December 2023 4,357 1 View
How does climate affect soil organic carbon and relationship between soil organic matter and organic carbon?
29 November 2023 6,640 7 View
What happens to most incoming solar radiation and how much of the incoming solar energy is absorbed by the atmosphere and clouds?
17 November 2023 523 5 View
Hello, I am performing compositional analysis of biochar (using ICP-OES), I've used digestion in using Nitric Acid (HNO3) and Hydrofluoric Acid (HF). The extract that I've obtained is not...
08 November 2023 7,519 0 View
What is seed biopriming with plant growth promoting rhizobacteria and role of plant growth promoting rhizobacteria PGPR in mitigating plants environmental stresses?
03 November 2023 1,335 0 View
Does a plant growth promoting rhizobacteria enhance agricultural sustainability and why are rhizobacteria important to plant roots?
03 November 2023 7,777 0 View
meiosis role in polyploidy cotton
28 October 2023 3,468 0 View
Dear people! I am in the process of planning my graduate thesis, and I'm considering revisiting the research topic I explored during my undergraduate studies. I want to know how closely I can...
26 October 2023 603 0 View
salt tolerance in cotton
24 October 2023 1,874 0 View
What are the 7 Rs of sustainable living and steps that need to be taken to promote sustainable agriculture and food security in the wake of climate change?
20 October 2023 5,952 3 View
Do millets stop calcium absorption and how do you remove phytic acid from millet and reduce anti-nutrients in millets and right way to eat millets?
17 October 2023 1,903 0 View
Are millets rich in fibre and which millet is best for constipation and who should not eat millets?
17 October 2023 6,135 0 View
Can millets be eaten at night and should we discard the water after soaking millets and ratio of water to millet?
17 October 2023 7,600 0 View
Do millets require pesticides and methods of controlling weeds in millets and what is the carbon footprint of millet?
17 October 2023 9,233 0 View
In many litratures, I found the allosteric site in TEM-1 is H10 helix , but the explination of how this allosteric site affected by the inhibitors sounds mysterious to me. Can you please...
06 October 2023 5,483 1 View
Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...
04 October 2023 2,796 3 View
Kindly contact me if you are willing to participate ([email protected]). Your participation will be greatly appreciated.
29 September 2023 6,930 0 View
Hello everyone, I'm following a protocol in which 1 ml of diethyl ether is used as a triturating solvent. The product should remain a solid while the impurities should dissolve in the diethyl...
22 September 2023 8,788 4 View
I am trying to quantify 5mC and 5hmC using Epimark 5mC and 5hmC kit from NEB. I did the end-point PCR after glucosylation and digestion with MspI and HpaII. It didnt generate a a yes or no answer,...
07 September 2023 6,090 0 View
The graph that I made on analysis of measurement is correct or not according to ISO definitions? and if it is not correct could someone post a single graph of probability distribution function...
07 September 2023 4,029 0 View
I'm looking for a psychometric regarding perception of PrEP and sexual expectancies that have items that also include attitudes/perception of risky sexual behaviour (RSB), i.e., condoles sex,...
25 August 2023 3,898 0 View