4 Questions 10 Answers 0 Followers
Questions related from Ziaur Rehman
Hi every body, I am working on mice sertoli cells. But I have some confusion in counting age of these cells. I kill the mice, collect the sertoli cells and culture on the same day(7th day). But...
04 April 2016 1,025 3 View
Hi every body, I have the following queries please guide me in simple and step wise, 1) Which type of gene is required for knowdown experiment in cells, I mean how to select a gene? 2) How to...
04 April 2016 5,779 4 View
Hello Can anyone tell me how to do an overexpression of a gene in sertoli cells. I used construct a EGFP-N1 plasmid but it doesn't show GFP in sertoli cells. I need an experts opinion in this...
09 September 2015 2,446 0 View
I want to use polybrene @6ug/ml for cells. But Sigma Alrich provides it as in 5grams packing. so can any one tell me step wise and in simple language how to dilute it ?
07 July 2015 5,476 5 View
