Zhanna Orlova

2 Questions 5 Answers 0 Followers

Questions related from Zhanna Orlova

Why interpretation of gene expression (qPCR) depends on reference gene used?

I am using siRNA knock down to evaluate changes in gene expression in cancer cells. I found that GAPD is an unsuitable reference gene for my system given that it highly variates between...

11 November 2019 2,249 5 View

Anyone culturing OVCAR-4 cell line can give specifications on the media?

I am culturing OVCAR-4 cells in RMPI 10% FBS and 1% pen/strep and supl with glut and HEPES the cell viability is low.

05 May 2018 7,212 3 View