Zaynab Hani El-Gammal

4 Questions 2 Answers 0 Followers

Questions related from Zaynab Hani El-Gammal

Alkaline phosphatase Pluripotency or differentiation?

I used live alkaline phosphatase to prove that the adult stem cells were reprogrammed to more pluripotent cells. This was accompanied by a significantly lower expression of 3-germ layers...

22 August 2020 4,678 3 View

Ultra-low attachment plate is sufficient for EB formation?

If I cultured pluripotent cells on ultra-low attachment plate in order to form Embryoid Bodies, and after 2 weeks I isolated the RNA from these cells and I found that the differentiation gene...

06 March 2019 3,706 0 View

Can I conclude cell cycle from Annexin PI staining?

I stained cells with Annexin PI and when I ploted PI va Annexin, I determined the early apoptosis, late apoptosis, and necrotic cells. Can I make a histogram for PI and conclude the cell cycle...

07 November 2018 4,002 2 View

Relative quantification between treated and untreated taking into consideration the treatment?

I am treating certain type of cells by cellular extract of another cell. Since this cellular extract (treatment) contain mRNA of the genes of interest, I want to compare the expression of the...

01 August 2018 7,749 1 View