Zakhar Krekhno

3 Questions 2 Answers 0 Followers

Questions related from Zakhar Krekhno

How much to dilute the fecal samples for detection of LPS response?

I am currently trying to analyze ROS response of cultured cells (HT-29) to mice feces. I homogenize 1 mouse poop in 2 ml of DMEM, split in half and filter sterilize 1 ml and heat kill and filter...

21 April 2019 8,151 0 View

Why would microscope images taken with DIC would look the same as brightfield?

We are using Leica DM5500b microscope to do our microscopy on Giardia intestinalis. However, despite the fact that around 3 years ago we were able to obtain amazing DIC pictures, the DIC pictures...

31 March 2018 4,637 3 View

Why do cells fall off the coverslip, when I do an IFA on them?

I work with Giardia intestinalis and I'm trying to perform an IFA on those cells, but no matter how many cells I pipette on the coverslip they tend to fall off, and I end up with very few cells,...

06 July 2017 4,690 2 View