Yuka Obayashi

8 Questions 10 Answers 0 Followers

Questions related from Yuka Obayashi

How long and what percentage of PFA should we use to fix postmortem human brain sample in a cassette for IF staining?

I recently received postmortem (aged) human brain samples in a cassette (previously fresh frozen) from brain bank. I would like to use those samples for fluorescent immunostaining. Could anyone...

01 October 2020 1,992 2 View

Are there any reliable antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus?

I have Camk2a-Cre mice model, and would like to have antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus CA1 regions. Do you know any good antibodies for...

08 November 2018 5,143 3 View

Can you suggest me articles/any websites/softwares that show single cell RNA seq data for Camkiia-Cre?

I would like to answer following questions: - the specific area of brain that Camkiia-Cre would be active in - specific cell types that the KO will occur in - figure out for each cell type (major...

29 July 2018 3,022 0 View

Any suggestible breeding scheme for generating Camkiia-Cre transgenic mice model?

In order to knockout our gene of interest (GOI) specifically in the hippocampal pyramidal cells (CA1), we set up breeding pairs Homozygous Cre X Homozygous Floxed mice (both male Cre x female...

29 July 2018 3,602 5 View

Which melting temperature and stability of agar/agarose should be used for embedding mouse brain tissue (for vibratome sectioning)?

It would be very helpful if you could suggest me with specific product# and required concentration. Also, reference might be helpful. The sections will be used for free floating...

27 July 2018 5,470 9 View

Do you have any suggestions for neurosphere assay protocol, using adult mice?

Do you have any suggestions for growth factors (EGF and FGF) effective/useful for proliferation medium? How do you sterilize surgical equipments - autoclave or buffer? Any tips to share -...

13 February 2018 2,318 2 View

Any (hippocampal) culture and cell line useful to investigate epigenetic regulation (ie. high proliferation rate)?

I am currently looking for (preferably hippocampus) culture/neurosphere/cell line with high proliferation rate, which are available to purchase in Canada.

13 February 2018 3,098 2 View

Are there any rodent behavioural experiments that can substitute contextual fear conditioning test?

I am investigating the role of histone modification during hippocampal memory formation. It is the best if I could perform contextual fear conditioning on mice. However, we unfortunately do not...

01 January 1970 8,149 1 View