Yue Lu

5 Questions 13 Answers 0 Followers

Questions related from Yue Lu

Has anyone fed pregnant mice with BrdU in drinking water?

I plan to lable the proliferating cells in E10-E16 mouse embryos with BrdU. So I fed pregnant females (C57BL/6) with BrdU (0.8mg/ml) in drinking water during E10-E16. and then switch to normal...

03 March 2016 4,814 4 View

Does anyone have experience in visualizing eGFP mice?

Hello, I'm trying to visualize eGFP mice which have uptimum excitation wavelength at 488nm. It seems that there is no commercial flashlight or lamp that output this or similar wavelength. The...

07 July 2015 10,041 11 View

Does anyone know what anti-pax7 works in mice?

I'm working on the IHC in mouse skeletal muscle and tried mouse anti-Pax7 (DSHK). It turned out that unspecific background was really intense. I know using mouse hosted antibody to stain mouse...

05 May 2015 3,027 4 View

Does vector mouse to mouse IgG blocking reagent works with invitrogen Alexa fluor conjugated secondary antibodies?

Hi, I'm recently working on immunostaining for Pax7 in mouse muscle cryosections. The primary antibody is mouse IgG1 anti-Pax7 (DSHB) and it seems the only antibody working in mouse with a Mouse...

05 May 2015 662 2 View

Does anyone have a protocol for tendon cryosection?

I'm recently working on mouse tendon cryosection for immunostaining. But when I cut these tendon samples, they scattered so it's very hard to get intact sections. I've tried to changed the...

04 April 2015 6,190 7 View