Yomna S. Momen

5 Questions 8 Answers 0 Followers

Questions related from Yomna S. Momen

Why I have 2 bands at the expected molecular weight and why I have wide band?

hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...

23 May 2024 7,516 2 View

How to normalize a protein band to Housekeeping gene band using Biorad Image lab software?

Hello Everyone, After running the analysis of the bands using Biorad image lab software, I got values for adjusted volume and volumes for each band. Which volume (the adjusted or the second one)...

03 May 2024 2,690 3 View

What is the Estimated Protein concentration of 1 ml HMC3 cell lysate?

Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...

26 April 2024 4,561 3 View

How can i be sure that all rats drink equal amount of water when i use DENA (100 mg/L) in drinking water to induce HCC in 8 weeks?

is it done by oral gavage and what is the dose/ day?

20 November 2016 7,937 5 View

Can I load equal volumes of samples that have different protein concentrations for western blot?

Hello Everyone. I have samples that significantly vary in protein concentrations. can I load equal volumes of samples that have different protein concentrations on basis that later on I will...

01 January 1970 1,909 3 View