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Questions related from Yimeng Guan
Hi everyone, I found some adherent cells in my PBMC culture today. The PBMC came from a healthy donor, they were cryopreserved before using. These cells are staying in cRPMI (10% HI FBS) with 10...
28 October 2023 6,715 1 View
Hi everyone, Im going to do HLA typing on the PBMCs to match our cancer cell lines which have been HLA typed. Most of them carry A02:01 and A01:01, some of them carry A29:02 and A24:02. I have...
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Dear all, I have some cells that I want to stain for Annexin V, but they were already fixed by PFA. Can I still do immunofluorescence on Annexin V? Also, do I need any special buffer? As for...
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Hi everyone, I am doing 3D cell culture. I have seen random holes in the tumour spheroids. Instead of forming a big necrotic core in the centre, they had holes distributed throughout the...
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I am doing 3D tissue culture. These black dots show up in my spheroids from time to time, please take a view of the picture below. Does anybody know about these black dots?
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