Yasaman Naziri

4 Questions 1 Answers 0 Followers

Questions related from Yasaman Naziri

Why actin cannot be considered as a suitable reference gene for miRNA studies?

Why actin cannot be considered as a suitable reference gene for miRNA differential expression studies? I would like to know what are the best reference genes for miRNA studies under chilling...

17 March 2022 6,983 0 View

Can you guide me on how to work with MapIspector software?

I would like to study the location of genes on the chromosome. I would like to learn how to work with MapInspector software. Unfortunately, I have not found any practical guide in this regard. I...

04 March 2022 9,456 0 View

Should the isolated total RNA be diluted necessarily for cDNA synthesis? What is the best way for the measurement of the required RNA?

I'm studying on 5 miRNAs under chilling stress in tomato plants. I would like to know the best way for the calculation of required total RNA for cDNA synthesis. I have obtained the concentration...

28 February 2022 7,973 4 View

Do you see anything unscientific or any abnormality in the process of doing this quantitative real-time analysis?

I am studying 5 microRNAs under chilling stress in tomato leaves. I am going to investigate the differential expression of these genes exposed to stress in comparison with normal conditions. I am...

21 February 2022 4,819 2 View