Yarden Shor Nareznoy

3 Questions 3 Answers 0 Followers

Questions related from Yarden Shor Nareznoy

How to measure width of epidermis from IF confocal pics?

Hey all! I'm trying to measure the width of the epidermis in skin sections from IF confocal pictures. Can anyone recommend a program / tips on how to do it ? Thanks.

23 December 2021 6,202 1 View

How to create stable cell line expressing big protein?

I want to create a stable cell line expressing a big protein (~6500bp). I have approx. 12kbp between the 3-5' LTR seq of a lentivirus expression plasmid. Has anyone succeeded doing so with...

17 November 2020 8,563 1 View

I'm isolating endothelial cells from spleen using FACS. I get around 300,000 cells per spleen but can't get any good RIN, any suggestions ?

Hello, we are using anti CD45+TER119 magnetic beads in order to get a clean endothelial cells fraction. we tried various protocols for RNA isolation, and we think that there might be a problem...

26 September 2016 7,452 4 View