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Questions related from Yang Zhang
Hi everyone: I am wondering can I use a multicopy gene as the reference gene for qPCR? If not, what are the potential problems involved? Thank you!
01 January 2020 3,198 4 View
Hi all, I use Q5 NEB high-fidelity polymerase for PCR. I usually do 4 replicates, and ethanol precipitate the PCR products (0.1 sample volume 3 M NaAc, 2 sample volume of ethanol). I got a big...
09 September 2019 5,644 3 View
Does a single phosphorylation site have an effect on protein mobility on SDS-PAGE gel？is that due to change in size, or charge? Thank you all for your thoughts :)
04 April 2019 744 3 View
I have a protein of interest. Let's call it protein X. I treat the cell extract with calf intestinal phosphatase (from NEB). incubate at 37 degree for 1 h. Protein X would have increased mobility...
04 April 2019 577 4 View
Hi All, Pol II RPB1 CTD is phosphorylated, and can be detected as doublet bands on gel. I expressed a Myc tagged Rpb1 in cell, and tried to detect Rpb1-Myc. However, I can only see one band with...
01 January 1970 373 5 View
