Yang Leo

6 Questions 4 Answers 0 Followers

Questions related from Yang Leo

How can I dtermine the proportion of ssDNA in samples?

Hi everyone, I want to evaluate the proportion of ssDNA in my sample, is there a easy and accurate way to do that, thank you so much for all your help!

17 May 2022 9,372 3 View

Does reduced AMPure XP bead ratio (0.7X ) affect the data quality?

To all the brilliant scientists out there, Recently I've been performing single cell DNA library for NGS illumina, and there was an issue that bothers me for weeks, I wonder has anyone...

24 April 2022 4,387 1 View

About the "good buffer"-MES buffer

I need to use MES buffer in my experiment, and I want them to be DNase free, can I UV sterilize the buffer without changing their properties?

01 January 1970 6,998 5 View

Will library clean-up using a bead ratio of 0.7X affect data quality of my library?

To all the brilliant scientists out there, Recently I've been performing single cell DNA library for NGS illumina, and there was an issue that bothers me for weeks, I wonder has anyone...

01 January 1970 3,290 0 View

Dilution of MES buffer and pH adjustment

Hi all, Now I have a MES buffer (1M, pH3), but I want MES buffer(0.2M, pH6) for my experiment, the only thing I know: I'll need to dilute the MES buffer first and then use NaOH solution to adjust...

01 January 1970 2,822 0 View

TET triple knockout (TKO) mESCs purchase

Hi everyone, I want to buy some TET TKO mESCs, but it seems all the biotechnologies only have wt ESCs available, do you have any recommendations？ Thank you very much!

01 January 1970 4,630 0 View