Yana Ilieva

5 Questions 2 Answers 0 Followers

Questions related from Yana Ilieva

What animals are "kids" in animal husbandry? More precisely, in "Hess, W.R. African Swine Fever Virus". Is it a kit of the mentioned rabbits?

I know the word to be for human children but Hess writes in 1971: "Others reported substantial attenuation after 100 passages in rabbits (MENDES, 1962). Another attenuated lapinized strain of ASF...

08 August 2023 4,604 1 View

Can I crop the controls of a PCR to pieces of gel that do not have controls?

Our gel with too combs is too big for the imager so we cut it into four parts. Now only one piece has a positive and negative control (but the run and electrophoresis are the same). Can I crop the...

08 June 2023 9,007 1 View

How long do blocking buffer and marking buffer for western blot last at 4°C?

After getting them out of the freezer (-20) and melting them in the fridge how long can the buffers (which contain skimmed milk) be stored there before use? Thanks!

11 August 2017 720 3 View

I forgot my lysed probes for western blot for 4 hours at room temperature. Have they deteriorated? Thanks.

Thank you in advance

26 July 2017 8,798 2 View

What does increase of the proapoptotic protein Bax in some tumor cell lines and decrease in others (after treatment with a cytotoxic substance) mean?

Does that mean that there`s apoptosis where an increase is found (in the means of western blot after treating ) and vice versa? Or can I speculate that the decrease is due to cellular resistance...

06 March 2017 743 4 View